产品详情
产品名称Cyclin F Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human
特异性The antibody detects endogenous levels of total Cyclin F protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human Cyclin F.
基因/蛋白名称Cyclin F
标记Unconjugated
别名FBX1; FBXO1; F-box only protein 1;
数据库入口号Swiss-Prot: P41002
NCBI Gene ID: 899
Uniprot
P41002
实际分子量88kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin F antibody #33352.
Western blot analysis of extracts from HeLa cells, using Cyclin F antibody #33352.
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Cyclin F antiobdy #33352. The lane on the left is treated with systhesized peptide.
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.
Tsz Kan Fung, J. Biol. Chem., Sep 2002; 277: 35140 - 35149.
Michael T. Tetzlaff, Mol. Cell. Biol., Mar 2004; 24: 2487 - 2498.
Cain H. Yam, Mol. Cell. Biol., Jan 1999; 19: 635 - 645.
Michael T. Tetzlaff, PNAS, Mar 2004; 101: 3338 - 3345.
如果您使用该产品33352发表了文章，请通知我们，让我们可以引用您的文献。
et al,Longikaurin A, a natural ent-kaurane, induces G2/M phase arrest via downregulation of Skp2 and apoptosis induction through ROS/JNK/c-Jun pathway in hepatocellular carcinoma cells.In Cell Death Dis on 2014 Mar 20 by Y-J Liao, H-Y Bai et al..PMID:24651440
, (2014),
PMID:
24651440
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