产品详情
产品名称Cytochrome c Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total Cytochrome c protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human Cytochrome c.
基因/蛋白名称Cytochrome c
标记Unconjugated
别名CYCS; CYC;
数据库入口号Swiss-Prot: P99999
NCBI Gene ID: 54205
Uniprot
P99999
实际分子量15kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from COS7 cells, using Cytochrome c antibody #33354.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using Cytochrome c antibody #33354.
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
R. Pullerits, Rheumatology, Jan 2005; 44: 32 - 39.
Bogdan Polevoda, J. Biol. Chem., Jun 2000; 275: 20508
Larry A. Allen, J. Biol. Chem., Jan 1995; 270: 110.
Akemi MATSUSHIMA, J. Biochem. (Tokyo), Jan 1986; 100: 543 - 551.
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et al,Activation of Fas death receptor pathway and Bid in hepatocytes is involved in saikosaponin D induction of hepatotoxicity.In Environ Toxicol Pharmacol on 2016 Jan by Feng Zhang , Li Chen et al..PMID:26645133
, (2016),
PMID:
26645133
et al,Grape seed proanthocyanidin extract ameliorates cisplatin-induced testicular apoptosis via PI3K/Akt/mTOR and endoplasmic reticulum stress pathways in rats. In J Food Biochem on 2021 Jun 21 by Xuhong Chang, Minmin Tian,et al..PMID:34152018
, (2021),
PMID:
34152018
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