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iNOS Antibody#33424

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产品详情

产品名称iNOS Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC IF

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous levels of total iNOS protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from human iNOS.

基因/蛋白名称iNOS

标记Unconjugated

别名NITRIC OXIDE SYNTHASE; INDUCIBLE; NITRIC OXIDE SYNTHASE; MACROPHAGE; NOS2A

数据库入口号Swiss-Prot: P35228
NCBI Gene ID: 4843

Uniprot P35228

实际分子量130kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using iNOS antibody #33424.
Immunofluorescence analysis of HepG2 cells, using iNOS antibody #33424.
Western blot analysis of extracts from Hela cells, using iNOS antibody #33424.
Western blot analysis of extracts from JK cells (Lane 2), using iNOS antiobdy #33424. The lane on the left is treated with synthesized peptide.

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Rachel J. Jones, Am J Physiol Heart Circ Physiol, Jun 2007; 292: H2634 - H2642.
Katrin Linker,Nucleic Acids Res., Aug 2005; 33: 4813 - 4827.
Donna-Marie McCafferty,Donna-Marie McCafferty,
Lian-Qun Qiu, J. Am. Soc. Nephrol., Aug 2004; 15: 2066 - 2078.

如果您使用该产品33424发表了文章，请通知我们，让我们可以引用您的文献。

et al,The Hippo-YAP signaling pathway drives CD24-mediatedimmune evasion in esophageal squamous cell carcinoma viamacrophage phagocytosis. In Oncogene on 2024 Feb by Xiaofeng Zhou, Ziyi Yan,et al..PMID:38168654 , (2024),
PMID: 38168654
et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy. , (2025),
PMID: 40122152

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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