产品详情
产品名称PCNA Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total PCNA protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human PCNA.
基因/蛋白名称PCNA
标记Unconjugated
别名cyclin; proliferating cell nuclear antigen;
数据库入口号Swiss-Prot: P12004
NCBI Gene ID: 5111
Uniprot
P12004
实际分子量36kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PCNA antibody #33468.
Western blot analysis of extracts from HepG2 cells, using PCNA antibody #33468.
Western blot analysis of extracts from Hela cells (Lane 2), colo cells(Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5) using PCNA antiobdy #33468. The lane on the left is treated with synthesized peptide.
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Anje Sporbert, Nucleic Acids Res., Jun 2005; 33: 3521 - 3528.
Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007; 292: L716 - L724.
Basavaraj Bagewadi, J. Virol., Nov 2004; 78: 11890 - 11903.
T Iida, I Suetake, Genes Cells, Oct 2002; 7: 997 -
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et al,Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5'tRFAla/DVL/β-catenin pathway inhibition.
, (2025),
PMID:
40128187
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