产品详情
产品名称Dyskerin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total Dyskerin protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from Internal of human Dyskerin.
基因/蛋白名称Dyskerin
标记Unconjugated
别名CBF5 homolog; DKC1; EC 5.4.99.-; Nucleolar protein NAP57;
数据库入口号Swiss-Prot: O60832
NCBI Gene ID: 1736
Uniprot
O60832
实际分子量57kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Western blot analysis of extracts from HeLa cells, using Dyskerin antibody #33605.
Immunofluorescence analysis of HeLa cells, using Dyskerin antibody #33605.
Isoform 1:Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.Isoform 3:Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
Heiss N.S., Nat. Genet. 19:32-38(1998).
Knight S.W., Am. J. Hum. Genet. 65:50-58(1999).
Hassock S., Genomics 55:21-27(1999).
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et al,H/ACA snoRNP Gene Family as Diagnostic and Prognostic Biomarkers for Hepatocellular Carcinoma. In Pharmgenomics Pers Med on 2021 Oct 19 by Mi Zhang, Wei Zhao,
et al..PMID:34703278
, (2021),
PMID:
34703278
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