产品详情
产品名称Histone H2AX Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IF
种属反应性Human
特异性The antibody detects endogenous levels of total Histone H2AX protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from internal of human Histone H2AX.
基因/蛋白名称Histone H2AX
标记Unconjugated
别名H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
数据库入口号Swiss-Prot: P16104
NCBI Gene ID: 3014
Uniprot
P16104
实际分子量15kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody #33686.
Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody #33686.
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).
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et al,ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human nasopharyngeal carcInoma. In Cancer Manag Res on 2019 Jan 9 by Zhang B, Cui B, et al..PMID:30666155
, (2019),
PMID:
30666155
et al,Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.In Oral Oncol on 2011 Mar by Li XH, Kikuchi K,et al..PMID:21310648
, (2011),
PMID:
21310648
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