产品详情
产品名称MED1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Human;Mouse
特异性The antibody detects endogenous levels of total MED1 protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from internal of human MED1.
基因/蛋白名称MED1
标记Unconjugated
别名Mediator complex subunit 1;
数据库入口号Swiss-Prot: Q15648
NCBI Gene ID: 5469
Uniprot
Q15648
实际分子量170kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells, using MED1 antibody #33902.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MED1 antibody #33902.
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Drane P., Oncogene 15:3013-3024(1997).
Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
Naeaer A.M., Nature 398:828-832(1999).
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