产品详情
产品名称LAMA5 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB;IHC;IF
种属反应性Human;Mouse
特异性The antibody detects endogenous levels of LAMA5 protein.
免疫原类型Peptide
免疫原描述The antiserum was produced against synthesized peptide derived from human LAMA5.
基因/蛋白名称LAMA5
标记Unconjugated
别名 Laminin subunit alpha-5; KIAA0533; KIAA1907;
数据库入口号Swiss-Prot: O15230
NCBI Gene ID: 3911
Uniprot
O15230
实际分子量400kd
浓度1.0mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000. IHC: 1:100-300 IF 1:100-300
Western Blot analysis of RAW HepG2 A549 cells
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523)Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Deloukas P., ure 414:865-871(2001).
Nagase T., DNA Res. 8:179-187(2001).
Nagase T., DNA Res. 5:31-39(1998)
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