产品详情
产品名称BLM Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by antigen-affinity chromatography.
应用IF WB
种属反应性Human
特异性The antibody detects endogenous levels of total BLM protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant fragment corresponding to a region within amino acids 30 and 385 of BLM.
基因/蛋白名称BLM
标记Unconjugated
别名BS antibody; MGC126616 antibody; MGC131618 antibody; MGC131620 antibody; RECQ2 antibody; RECQL2 antibody; RECQL3 antibody; BLM antibody; "DNA helicase; RecQ-like type 2 antibody"; Bloom syndrome protein antibody; recQ protein-like 3 antibody; "Bloom syndr
数据库入口号Swiss-Prot#:P54132;
NCBI Gene#:641
Uniprot
P54132
实际分子量159kd
浓度0.93mg/ml
配方Rabbit IgG in 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C
应用详情
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:1000
BLM antibody detects BLM protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
#35370 diluted at 1:500
BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLM protein stained by BLM antibody, N-term (#35370) diluted at 1:500.
Blue: Hoechst 33342 staining.
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq]
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et al,Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe
, (2024),
PMID:
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