产品详情
产品名称ACTB Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification.
应用WB
种属反应性Human;Mouse
特异性The antibody detects endogenous levels of total ACTB protein.
免疫原类型Peptide
免疫原描述Synthetic peptide corresponding to residues near the N terminal of human Actin, beta
基因/蛋白名称ACTB
标记Unconjugated
别名BRWS1; PS1TP5BP1
数据库入口号Swiss-Prot#: P60709
NCBI Gene ID: 60
Gene Accssion: NP_001092
Uniprot
P60709
实际分子量42kd
浓度1.6mg/ml
配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:1000-1:5000
Gel: 10%SDS-PAGE
Lysates (from left to right): Hela, Jurkat and NIH/3T3 cell, human fetal liver and mouse brain tissue, A431 and 293T cell
Amount of lysate: 40ug per lane
Primary antibody: 1/600 dilution
Secondary antibody dilution: 1/8000
Exposure time: 7 seconds
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.
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