产品详情
产品名称HA Tag Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification.
应用WB
特异性The antibody detects transfected proteins contanining HA tag.
免疫原类型Peptide
免疫原描述Synthetic peptide: YPYDVPDYA (Human influenza hemagglutinin-HA-epitope) conjugated to KLH.
基因/蛋白名称HA Tag
标记Unconjugated
别名HA epitope tag; Hemagglutinin Tag
实际分子量55kd
浓度0.5mg/ml
配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500-1:5000
Gel: 8%SDS-PAGE
Lysate: 2ug Recombinant HA tagged protein
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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