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HA Tag Antibody#35534

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产品详情

产品名称HA Tag Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antigen affinity purification.

应用WB

特异性The antibody detects transfected proteins contanining HA tag.

免疫原类型Peptide

免疫原描述Synthetic peptide: YPYDVPDYA (Human influenza hemagglutinin-HA-epitope) conjugated to KLH.

基因/蛋白名称HA Tag

标记Unconjugated

别名HA epitope tag; Hemagglutinin Tag

实际分子量55kd

浓度0.5mg/ml

配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500-1:5000
Gel: 8%SDS-PAGE
Lysate: 2ug Recombinant HA tagged protein
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

如果您使用该产品35534发表了文章,请通知我们,让我们可以引用您的文献。

et al,Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.In Mol Cell on 2017 Mar 2 by Xu Qian , Xinjian Li,et al..PMID: 28238651 , (2017),
PMID: 28238651
et al,PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1 , (2019),
PMID: 31492635

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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