产品详情
产品名称c-Myc Tag Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification.
应用WB
特异性The antibody detects transfected proteins contanining c-Myc Tag.
免疫原类型Peptide
免疫原描述Synthetic peptide: C-EQKLISEEDL conjugated with KLH.
基因/蛋白名称c-Myc Tag
标记Unconjugated
别名c- Myc epitope Tag
Uniprot
P01106
实际分子量55kd
浓度1mg/ml
配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:1000-1:5000
Gel: 10%SDS-PAGE
Lysate:2ug Recombinant HA tagged protein
Primary antibody: 1/1000 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
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