Yes
Gel: 8%SDS-PAGE
Lysate: 20ug GFP transfected 293T cell
Primary antibody: 1/800 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Lysate: 20ug GFP transfected 293T cell
Primary antibody: 1/800 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Gel: 8%SDS-PAGE
Lysates: 20ug GFP transfected 293T cell per lane
Primary antibody: at various dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Lysates: 20ug GFP transfected 293T cell per lane
Primary antibody: at various dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
