产品详情
产品名称PCNA Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IHC
种属反应性Human;Mouse
特异性Antibody can detect endogenous?PCNA protein.
基因/蛋白名称PCNA
标记Unconjugated
Uniprot
P12004
实际分子量36kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:5000
Immunohistochemistry: 1:200
Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4), using #37978 diluted at 1:5000.
IHC staining of paraffin- embedded Human breast cancer tissue with PCNA mouse mAb (12D10) diluted at 1:200.
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
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