产品详情
产品名称Histone H3 Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IF IP
种属反应性Human;Mouse;Rat
特异性The Histone H3 antibody can detects endogenous Histone H3 protein.
基因/蛋白名称Histone H3
标记Unconjugated
别名H3/A; H3FA;H3FB; H3FC
数据库入口号Swiss-Prot#:P68431
Uniprot
P68431
实际分子量17kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:2000~1:5000
Immunofluorescence: 1:100-500
Immunoprecipitation: 1:200
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using #37988 diluted at 1:5,000.
IF analysis of Hela with DAPI (Left) and #37988(Right) diluted at 1:200.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #37988 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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