产品详情
产品名称CD68 Mouse Monoclonal Antibody
克隆号6F3
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用IHC,IF
种属反应性Hu Ms Rt
特异性The CD68 antibody detects endogenous CD68 proteins.
基因/蛋白名称CD68
标记Unconjugated
别名CD68; CD68 antigen; CD68 molecule; DKFZp686M18236; Gp110; macrophage antigen CD68
数据库入口号Swiss-Prot#:P34810
Uniprot
P34810
实际分子量37kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
IHC dilution: 1:200
IF dilution:1:50-200
IHC staining of paraffin-embedded Human tonsil tissue CD68 mouse mAb(6F3) diluted at 1:200.
Immunofluorescence analysis of Human-lung-cancer tissue. 1,CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,CD68 Monoclonal Antibody(6F3) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CD68 Monoclonal Antibody(6F3) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-spleen tissue. 1,CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
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