产品详情
产品名称GFAP Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
应用WB IHC IF
种属反应性Human，Rat，Mouse
特异性The GFAP Mouse Monoclonal antibody detects endogenous GFAP proteins.
基因/蛋白名称GFAP
标记Unconjugated
别名FLJ45472; GFAP; Glial fibrillary acidic protein
数据库入口号Swiss-Prot#:P14136
Uniprot
P14136
实际分子量45kd
浓度1.0mg/ml
配方PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
保存Store at -20˚C
应用详情
WB: 1:2000~1:5000
IHC: 1:50-300
IF: 1:100-1:200
Western blot analysis of lysates from 1) Rat Brain Tissue, 2)HeLa , 3)A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Rat-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],
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