产品详情
产品名称Caspase 9 Mouse Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IP
种属反应性Human;Mouse;Rat
特异性Antibody detects endogenous Caspase 9 protein.
基因/蛋白名称Caspase 9
标记Unconjugated
别名APAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6
数据库入口号Swiss-Prot#:P55211
Uniprot
P55211
实际分子量46kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:1000~1:5000
Immunoprecipitation: 1:200
Western blot analysis of Hela cells, using #38034 diluted at 1£©1:2,000 2£©1:5,000
Western blot analysis of 1£©Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart, using #38034 diluted at 1:3,000.
1¡¢Input: Hela Cell Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #38034 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
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et al,IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo
, (2014),
PMID: