产品详情
产品名称β-actin Mouse Monoclonal Antibody
克隆号5B7
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IHC IF
种属反应性Hu Rt Ms Mk Dg Chk Hm Rb Pg Sh
特异性Antibody detects endogenous β-actin protein.
基因/蛋白名称β-actin
标记Unconjugated
别名ACTB; BRWS1; PS1TP5BP1
数据库入口号Swiss-Prot#:P60709
Uniprot
P60709
实际分子量45kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:200
Western blot analysis of 1) 293T ,2) Mouse Kidney tissue, 3) Hela, 4) Rat Heart tissue, 5) Rat Brain tissue, using #38074 diluted at 1:5,000.
IHC staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200.
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
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et al,Gut-Flora-Dependent Metabolite Trimethylamine-N-Oxide Promotes Atherosclerosis-Associated Inflammation Responses by Indirect ROS Stimulation and Signaling Involving AMPK and SIRT1. In Nutrients on 2022 Aug 15 by Sa Zhou, Jiamin Xue, et al..PMID:36014845
, (2022),
PMID:
36014845
et al,Novel oxicam nonsteroidal compound XK01 attenuates inflammation by suppressing the NF-κB and MAPK pathway in RAW264.7 macrophages. In Heliyon on 2024 Jan 9 by Jixiang Wang, Jiawang Tan,et al..PMID:38312593
, (2024),
PMID:
38312593
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