产品详情
产品名称β-tubulin Mouse Monoclonal Antibody
克隆号5G3
来源种属Mouse
克隆性Monoclonal
纯化Affinity purification using immunogen.
应用WB IHC IF
种属反应性Hu Rt Ms Mk Dg Chk Hm Rb Sh
特异性Antibody detects endogenous β-tubulin protein.
基因/蛋白名称β-tubulin
标记Unconjugated
别名beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5
数据库入口号Swiss-Prot#:P07437
Uniprot
P07437
实际分子量55kd
浓度1.0mg/ml
配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:200
Western blot analysis of 1) Hela ,2) Mouse Kidney tissue, 3) Rat Heart tissue, using #38075 diluted at 1:5,000.
IHC staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
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et al,The effect of exposure time and concentration of airborne PM2.5 on lung Injury In mice: A transcriptome analysis. In Redox Biol on 2019 Sep by Wang H, Shen X et al..PMID:31279222
, (2019),
PMID:
31279222
Yes
