产品详情
产品名称IRS1 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total IRS1 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human IRS1.
基因/蛋白名称IRS1
标记Unconjugated
别名IRS1;HIRS-1;
数据库入口号Swiss-Prot#: P35568
NCBI Gene ID: 3667
Uniprot
P35568
实际分子量132kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using IRS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IRS1 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of HepG2 cells, using IRS1 antibody at 1:1000 dilution.
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
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et al,Effects of periodontitis on aortic insulin resistance in an obese rat model.In Lab Invest on 2010 Mar by Daisuke Ekuni, Takaaki Tomofuji,et al..PMID:20065945
, (2010),
PMID:
20065945
et al,Alleviative effects of α-lipoic acid on muscle atrophy via the modulation of TNF-α/JNK and PI3K/AKT pathways in high-fat diet and streptozotocin-induced type 2 diabetic ratsInFood Sci NutrOn2023 Jan 12byChih-Yuan Ko 1 2 3, Chi-Hao Wu et al..PMID: 37051351
, (2023),
PMID:
37051351
Yes
