产品详情
产品名称CD3D antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Rat
特异性The antibody detects endogenous level of total CD3D protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human CD3D.
基因/蛋白名称CD3D
标记Unconjugated
别名CD3D;CD3-DELTA;T3D;
数据库入口号Swiss-Prot#: P04234
NCBI Gene ID: 915
Uniprot
P04234
实际分子量19kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Immunohistochemistry of paraffin-embedded rat spleen using CD3D antibody at dilution of 1:200 (40x lens).
Western blot analysis of extracts of Jurkat cells, using CD3D antibody at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
Immunofluorescence analysis of Jurkat cells using CD3D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
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et al,CD155 Cooperates with PD-1/PD-L1 to Promote Proliferation of Esophageal Squamous Cancer Cells via PI3K/Akt and MAPK Signaling Pathways. In Cancers (Basel) on 2022 Nov 15 by Xiyang Tan, Jie Yang, et al..PMID:36428703
, (2022),
PMID:
36428703
Yes
