产品详情
产品名称DDB1 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total DDB1 protein.
免疫原类型Peptide
免疫原描述A synthetic peptide of human DDB1.
基因/蛋白名称DDB1
标记Unconjugated
别名XPE;DDBA;XAP1;XPCE;XPE-BF;UV-DDB1;
数据库入口号Swiss-Prot#: Q16531
NCBI Gene ID: 1642
Uniprot
Q16531
实际分子量127kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDB1 antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded rat testis using DDB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDB1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
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et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity
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PMID: