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PARP1 antibody#38592

是否有货: Yes
产品详情

产品名称PARP1 antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC IF

种属反应性Human;Mouse

特异性The antibody detects endogenous level of total PARP1 protein.

免疫原类型peptide

免疫原描述Synthesized peptide derived from human PARP-1

基因/蛋白名称PARP1

标记Unconjugated

别名ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;

数据库入口号Swiss-Prot#: P09874
NCBI Gene ID: 142

Uniprot P09874

实际分子量113kd

浓度1.0mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C

应用详情
WB 1:500-1:2000;
IHC 1:50-1:200;
IF 1:50-1:200;
Western blot analysis of extracts of various cell lines, using PARP1 antibody.
Immunofluorescence analysis of A549 cell using PARP1 antibody. Blue: DAPI for nuclear staining.
Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

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et al,Anticancer activity and mechanism investigation of beauvericin isolated from secondary metabolites of the mangrove endophytic fungi.In Anticancer Agents Med Chem on 2015 by Yi-wen Tao, Yong-cheng Lin et al..PMID:25641103 , (2015),
PMID: 25641103
et al,Bruceine D induces apoptosis in human chronic myeloid leukemia K562 cells via mitochondrial pathway.In Am J Cancer Res on 2016 Mar 15 by Jian-Ye Zhang, Min-Ting Lin et al..PMID: 27186433 , (2016),
PMID: 27186433

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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