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位置: 首页 > KO/KD抗体 > AKT1S1 antibody

AKT1S1 antibody#38768

是否有货: Yes
产品详情

产品名称AKT1S1 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity purification using immunogen.

应用WB,IHC,IF

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of total AKT1S1 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant protein of human AKT1S1.

基因/蛋白名称AKT1S1

标记Unconjugated

别名Lobe; PRAS40;

数据库入口号Swiss-Prot#: Q96B36
NCBI Gene ID: 84335

Uniprot Q96B36

实际分子量27kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:2000

Western blot analysis of extracts of various cell lines, using AKT1S1 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1S1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AKT1S1 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using AKT1S1 Polyclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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