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Bcl-2 Monoclonal Antibody#40415

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产品详情

产品名称Bcl-2 Monoclonal Antibody

克隆号6B5

来源种属Mouse

克隆性Monoclonal

纯化The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

应用WB IHC IF/ICC

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous Bcl-2 proteins.

免疫原描述Synthetic peptide of human Bcl-2

基因/蛋白名称Bcl-2

标记Unconjugated

别名BCL2; Apoptosis regulator Bcl-2

数据库入口号Swiss-Prot: P10415
NCBI Gene ID: 596

Uniprot P10415

实际分子量26kd

浓度0.73mg/mL

配方PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:1000~2000
Immunohistochemistry: 1:200

IF: 1:50-200

Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA1
Western blot analysis of lysates from 1)Hela, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer.
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.
Immunofluorescence analysis of Hela cell. 1,Dnmt3b Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

如果您使用该产品40415发表了文章,请通知我们,让我们可以引用您的文献。

et al,Physalin B reduces tau phosphorylation and cell apoptosis in HEK293 cells by activating FoxO1. In Curr Mol Pharmacol on 2024 by Wei Zhang, Yating Shi,et al..PMID:37489791 , (2024),
PMID: 37489791
et al,IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo , (2014),
PMID:

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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