产品详情
产品名称ZNF281 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Human
特异性The antibody detects endogenous levels of total ZNF281 protein.
免疫原类型protein
免疫原描述Fusion protein of human ZNF281
基因/蛋白名称ZNF281
标记Unconjugated
别名ZBP-99; ZNP-99
数据库入口号Swiss-Prot#: Q9Y2X9
NCBI Gene ID: 23528
Uniprot
Q9Y2X9
计算分子量97kd
浓度1.6mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500-2000
Immunohistochemistry: 1: 100-300
Gel: 6%SDS-PAGE
Lysate: 40 μg, Lane: A549 cell,
Primary antibody:ZNF281 antibody at dilution 1/600 dilution,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ZNF281 Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ZNF281 Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200)
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF281, also known as GC-box-binding zinc finger protein 1, ZBP-99 or ZNP-99 (zinc finger DNA-binding protein 99), is a zinc finger protein that belongs to the Kr¨¹ppel C2H2-type zinc finger protein family. It is expressed ubiquitously at low levels with predominant expression in kidney, liver, lymphocytes and placenta. ZNF281 localizes to the nucleus and contains four C2H2-type zinc fingers. ZNF281 plays a role in repressing the transcription of a variety of genes including Gastrin and ODC (ornithine decarboxylase). In particular, ZNF281 functions by binding to the G-rich box in the enhancer region of the gene. Upon DNA damage, ZNF281 may become phosphorylated by Atm or ATR.
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et al,Cancer-associated fibroblasts facilitate premetastatic niche formation through lncRNA SNHG5-mediated angiogenesis and vascular permeability in breast cancer. In Theranostics on 2022 Oct 17 by Huan Zeng, Yixuan Hou, et al..PMID:36438499
, (2022),
PMID:
36438499
Yes
