产品详情
产品名称UBE2W Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Human;Rat
特异性The antibody detects endogenous levels of total UBE2W protein.
免疫原类型protein
免疫原描述Full length fusion protein
基因/蛋白名称UBE2W
标记Unconjugated
别名UBC16; UBC-16
数据库入口号Swiss-Prot#: Q96B02
NCBI Gene ID: 55284
Uniprot
Q96B02
计算分子量17kd
浓度1.4mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500-2000
Immunohistochemistry: 1: 100-250
Gel: 12%SDS-PAGE
Lysate: 40 μg, Lane 1-2: 231 cell and Human cerebrum tissue lysates,
Primary antibody:UBE2W antibody at dilution 1/550,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 4 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using UBE2W Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using UBE2W Antibody at dilution 1/100, on the right is treated with fusion protein. (Original magnification: x200)
This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.?
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et al,Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W
, (2023),
PMID:
36593310