产品详情
产品名称CEP72 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用WB IHC
种属反应性Human;Mouse
特异性The antibody detects endogenous levels of total CEP72 protein.
免疫原类型peptide
免疫原描述Synthetic protein corresponding to residues near the C terminal of human CEP72
基因/蛋白名称CEP72
标记Unconjugated
数据库入口号Swiss-Prot:Q9P209
NCBI Gene ID:55722
NCBI Protein:BC000132
Uniprot
Q9P209
计算分子量72 kDa
浓度1mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:200-1:1000
Immunohistochemistry: 1: 30-150
Gel: 6%SDS-PAGE
lysate: 40 ¦Ìg, Lane 1-3: TM4£¬NIH/3T3 and Jurkat cell lysates,
Primary antibody: 46478£¨CEP72 Antibody) at dilution 1/600
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46478(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46478(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM). Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. CEP72 (centrosomal protein 72kDa) is a 647 amino acid protein that localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. Involved in the recruitment of key centrosomal proteins to the centrosome, CEP72 provides centrosomal microtubule-nucleation activity on the ? Tubulin ring complexes and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. CEP72 exists as two alternatively spliced isoforms.
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et al,Increased expression of CEP72 predicts poor prognosis in multiple myelomaInInt J Lab HematolOn2023 JunbyDan Guo?#?1?2,?Jinfeng Lu et al..PMID:?36782078
, (2023),
PMID:
36782078
Yes
