产品详情
产品名称DYKDDDDK Tag(FLAG) Antibody
克隆号A2-A4
来源种属Mouse
克隆性Monoclonal
纯化ProG affinity purified
应用WB, IP
免疫原描述peptide
标记Unconjugated
别名DDDDK epitope tag antibody DDDK antibody ddk antibody DYKDDDDK antibody DYKDDDDK epitope tag antibody DYKDDDDK tag antibody ECS epitope tag antibody ECS tag antibody Enterokinase Cleavage Site epitope tag antibody Enterokinase Cleavage Site tag antibody FLAG antibody FLAG tag antibody
数据库入口号Swiss-Prot#:
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:5,000-1:10,000
IP: 1:10-1:50
Western blot analysis on DYKDDDDK Tag recombinant protein (65kDa).
Immunoprecipitation analysis of different lysates using anti-FLAG antibody. Lane 1: Non-transfected 293T Lane 2: Transfected 293T Lane 3: Agarose beads complexes Lane 4: Negative control (Normal mouse IgG)
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
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