产品详情
产品名称GRP78 BiP Antibody
克隆号C9-9
来源种属Mouse
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC, IHC
种属反应性Human;Mouse
免疫原描述recombinant protein
标记Unconjugated
别名78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody
数据库入口号Swiss-Prot#:P11021
Uniprot
P11021
计算分子量78kDa
配方1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:2,000-1:5,000
ICC: 1:50-1:100
Western blot analysis on cell lysates using anti- GRP78 mouse mAb.
ICC staining GRP78 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GRP78 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.
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et al,Sestrin2 Overexpression Ameliorates Endoplasmic Reticulum Stress-Induced Apoptosis via Inhibiting mTOR Pathway in HepG2 Cells. In Int J Endocrinol on 2022 Dec 10 by Huiling Hu, Zhijun Luo, et al..PMID:36536875
, (2022),
PMID:
36536875
Yes
