产品详情
产品名称Beta III Tubulin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Peptide affinity purified
应用WB, ICC, IHC, FC
种属反应性Human;Mouse;Rat
免疫原描述peptide
标记Unconjugated
别名beta 3 tubulin antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody
数据库入口号Swiss-Prot#:Q13509
Uniprot
Q13509
计算分子量50kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:2000-1:5000
ICC: 1:100-1:200
Western blot analysis on different lysates using anti-beta III tubulin rabbit polyclonal antibodies.
ICC staining beta III tubulin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. It has been reported that β3-tubulin is expressed in a wide variety of tumors. Overexpression of this isotype in clinical samples correlates with tumor aggressiveness, resistance to chemotherapeutic drugs, and poor patient survival. The β3 isotype increases tumor aggressiveness by two distinct mechanisms. Incorporation of this isotype makes microtubule networks hypostable, allowing them to resist the cytotoxic effects of microtubule stabilizing drugs like taxanes or epothilones. Mechanistically, it was found that overexpression of β3-tubulin increases the rate of microtubule detachment from microtubule organizing centers, an activity that is suppressed by drugs such as paclitaxel.
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