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Angiopoietin-2 Antibody #48274

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产品详情

产品名称Angiopoietin-2 Antibody

克隆号4A2

来源种属Mouse

纯化ProA affinity purified

应用WB, IP, IF, IHC

种属反应性Human;Mouse;Rat

免疫原描述peptide

标记Unconjugated

别名AGPT 2 antibody
Agpt2 antibody
ANG 2 antibody
ANG-2 antibody
ANG2 antibody
Angiopoietin 2a antibody
Angiopoietin 2B antibody
Angiopoietin-2 antibody
Angiopoietin2 antibody
ANGP2_HUMAN antibody
ANGPT 2 antibody
Angpt2 antibody
Tie2 ligand antibody

数据库入口号Swiss-Prot#:O15123

Uniprot O15123

计算分子量62-70kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at 4˚C

应用详情

WB: 1:100-1:1,000
IHC: 1:50-500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Western Blot analysis of Ang-2 expression in HUV-EC-C (A) and TF-1 (B) whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification.

Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases. The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three Fibronectin type III repeats next to the transmembrane region. Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential Fibrinogen-like domain at the carboxy-terminus and coiled-coil regions in the amino-terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling.

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操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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025-58868422

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