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BrdU (Bu20a) Antibody #48275

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产品详情

产品名称BrdU (Bu20a) Antibody

克隆号1G1

来源种属Mouse

纯化ProA affinity purified

应用IF, IHC(P), FCM

种属反应性Bromodeoxyuridine

免疫原描述peptide

标记Unconjugated

别名Bromodeoxyuridine antibody
BUdr antibody

数据库入口号Swiss-Prot#:

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at 4˚C

应用详情


IHC: 1:50-500


FC: 1 μg per 1 x 106 cells

Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.
Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.

The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

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应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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025-58868422

Email Address

info@sabbiotech.com

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