产品详情
产品名称E-Cadherin Antibody
克隆号A0-G11-2
来源种属Mouse
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC, IHC, FC
种属反应性Human;Mouse
免疫原描述recombinant protein
标记Unconjugated
别名Arc 1 antibody
CADH1_HUMAN antibody
Cadherin 1 antibody
cadherin 1 type 1 E-cadherin antibody
Cadherin1 antibody
CAM 120/80 antibody
CD 324 antibody
CD324 antibody
CD324 antigen antibody
cdh1 antibody
CDHE antibody
E-Cad/CTF3 antibody
E-cadherin antibody
ECAD antibody
Epithelial cadherin antibody
epithelial calcium dependant adhesion protein antibody
LCAM antibody
Liver cell adhesion molecule antibody
UVO antibody
Uvomorulin antibody
数据库入口号Swiss-Prot#:P09803
Uniprot
P09803
计算分子量130 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
IHC: 1:200
ICC: 1:200
FC: 1:50-1:100
Western blot analysis of E-cadherin on different cell lysates using anti- E-cadherin antibody at 1/1000 dilution. Positive control:
Lane 1: A431
Lane 2: SW480
Lane 3: MCF-7
Lane 4: Hela
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin.
ICC staining E-Cadherin in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HeLa cells with E-Cadherin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody
E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer.
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et al,ADAMTS13 attenuates renal fibrosis by suppressing thrombospondin 1 mediated TGF-β1/Smad3 activation.
, (2025),
PMID:
39929281
Yes
