免费电话:400-9961-933
邮箱:info@sabbiotech.com
欢迎光临本站
川博智能体 English
位置: 首页 > 内参抗体 > Beta-Actin Antibody

Beta-Actin Antibody#48485

是否有货: Yes
产品详情

产品名称Beta-Actin Antibody

克隆号A2-F6

来源种属Mouse

克隆性Monoclonal

纯化ProA affinity purified

应用WB, ICC, IHC, FC

种属反应性Human;Mouse;Rat

免疫原描述Synthetic peptide (KLH-coupled) within human Beta-actin N terminal.

标记Unconjugated

别名A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
Beta-actin antibody
BRWS1 antibody
E430023M04Rik antibody
MGC128179 antibody
PS1TP5 binding protein 1 antibody
PS1TP5BP1 antibody

数据库入口号Swiss-Prot#:P60709

Uniprot P60709

计算分子量42kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:10,000
IHC: 1:100-1:200
ICC: 1:100-1:200

FC: 1:100-1:200
Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.

Positive control:

Lane 1: NIH/3T3

Lane 2: PC12

Lane 3: MCF-7

Lane 4: HepG2

Lane 5: Hela

Lane 6: Mouse lung

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela?cells with Beta-actin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.

如果您使用该产品48485发表了文章,请通知我们,让我们可以引用您的文献。

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

info@sabbiotech.com