产品详情
产品名称Beta-Actin Antibody
克隆号A2-F6
来源种属Mouse
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC, IHC, FC
种属反应性Human;Mouse;Rat
免疫原描述Synthetic peptide (KLH-coupled) within human Beta-actin N terminal.
标记Unconjugated
别名A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
Beta-actin antibody
BRWS1 antibody
E430023M04Rik antibody
MGC128179 antibody
PS1TP5 binding protein 1 antibody
PS1TP5BP1 antibody
数据库入口号Swiss-Prot#:P60709
Uniprot
P60709
计算分子量42kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:10,000
IHC: 1:100-1:200
ICC: 1:100-1:200
FC: 1:100-1:200
Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: PC12 Lane 3: MCF-7 Lane 4: HepG2 Lane 5: Hela Lane 6: Mouse lung
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela?cells with Beta-actin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
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