Yes
Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody at 1/2,000 dilution.
Lane 1: Raji cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 3 minutes 54 seconds;
4-20% SDS-PAGE gel.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
