产品详情
产品名称Alas1 Rabbit mAb
克隆号SA70-04
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名5 aminolevulinate synthase antibody 5 aminolevulinate synthase nonspecific mitochondrial antibody 5 aminolevulinic acid synthase antibody 5-aminolevulinate synthase antibody 5-aminolevulinic acid synthase 1 antibody Alas 1 antibody ALAS 3 antibody ALAS antibody ALAS H antibody ALAS HOUSEKEEPING TYPE antibody ALAS N antibody ALAS-H antibody alaS1 antibody ALAS3 antibody ALASH antibody Aminolevulinate delta synthase 1 antibody Aminolevulinic acid synthase 1 antibody Delta ALA synthetase antibody Delta aminolevulinate synthase antibody Delta-ALA synthase 1 antibody Delta-aminolevulinate synthase 1 antibody HEM1_HUMAN antibody MIG 4 antibody MIG4 antibody Migration inducing protein 4 antibody mitochondrial antibody nonspecific antibody
数据库入口号Swiss-Prot#:P13196
Uniprot
P13196
计算分子量71 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
ICC: 1:50-1:200
Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution. Positive control: Lane 1: JAR Lane 2: HUVEC Lane 3: Hela
ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.
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