产品详情
产品名称Firefly Luciferase Rabbit mAb
克隆号SR03-86
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,ICC,IF
种属反应性Firefly
免疫原描述Recombinant protein
标记Unconjugated
别名ec 1.13.12.7 antibody
Firefly antibody
Luciferase antibody
Luciferin 4 monooxygenase antibody
数据库入口号Swiss-Prot#:P08659
Uniprot
P08659
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
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