产品详情
产品名称Renilla Luciferase Rabbit mAb
克隆号SR07-830
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,ICC,IF
种属反应性Sea pansy
免疫原描述Recombinant protein
标记Unconjugated
别名Renilla luciferin 2 monooxygenase antibody
Renilla type luciferase antibody
数据库入口号Swiss-Prot#:P27652
Uniprot
P27652
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
Western blot analysis of Renilla Luciferase on luciferase recombinant protein lysate using anti-Renilla Luciferase antibody at 1/1,000 dilution.
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
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