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Cdk2 Rabbit mAb #48670

是否有货: Yes
产品详情

产品名称Cdk2 Rabbit mAb

克隆号SR01-01

来源种属Recombinant Rabbit

克隆性Monoclonal

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名Cdc2 related protein kinase antibody
cdc2-related protein kinase antibody
CDC28 antibody
CDC2A antibody
Cdk 2 antibody
CDK1 antibody
CDK2 antibody
CDK2_HUMAN antibody
CDKN2 antibody
Cell devision kinase 2 antibody
Cell division protein kinase 2 antibody
Cyclin dependent kinase 2 antibody
cyclin dependent kinase 2-alpha antibody
Cyclin-dependent kinase 2 antibody
kinase Cdc2 antibody
MPF antibody
p33 protein kinase antibody
p33(CDK2) antibody

数据库入口号Swiss-Prot#:P24941

Uniprot P24941

计算分子量34 kDa

实际分子量34 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
ICC staining Cdk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: CDK2 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysates
Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 2 seconds
All lanes: CDK2 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat kidney lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 4 seconds
All lanes:CDK2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDK2 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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info@sabbiotech.com