产品详情
产品名称Cdk2 Rabbit mAb
克隆号SR01-01
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名Cdc2 related protein kinase antibody cdc2-related protein kinase antibody CDC28 antibody CDC2A antibody Cdk 2 antibody CDK1 antibody CDK2 antibody CDK2_HUMAN antibody CDKN2 antibody Cell devision kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody kinase Cdc2 antibody MPF antibody p33 protein kinase antibody p33(CDK2) antibody
数据库入口号Swiss-Prot#:P24941
Uniprot
P24941
计算分子量34 kDa
实际分子量34 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.
ICC staining Cdk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk2 in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: CDK2 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : 293 whole cell lysates Lane 3 : C6 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 34 kDa Observed band size: 34 kDaExposure time: 2 seconds
All lanes: CDK2 Rabbit mAb at 1/1k dilutionLane 1 : Mouse kidney lysates Lane 2 : Mouse brain lysates Lane 3 : Rat kidney lysates Lane 4 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 34 kDa Observed band size: 34 kDaExposure time: 4 seconds
All lanes:CDK2 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : CDK2 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.