产品详情
产品名称Caspase-8 Rabbit mAb
克隆号SZ01-08
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名ALPS2B antibody Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody Apoptotic cysteine protease antibody Apoptotic protease Mch-5 antibody Apoptotic protease Mch5 antibody CAP4 antibody CASP-8 antibody CASP8 antibody CASP8_HUMAN antibody Caspase 8 antibody Caspase 8 apoptosis related cysteine peptidase antibody Caspase-8 subunit p10 antibody CED 3 antibody FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease antibody FADD-like ICE antibody FLICE antibody FLJ17672 antibody ICE-like apoptotic protease 5 antibody MACH alpha 1/2/3 protein antibody MACH antibody MACH beta 1/2/3/4 protein antibody MCH5 antibody MGC78473 antibody MORT1 associated ced 3 homolog antibody MORT1-associated CED-3 homolog antibody OTTHUMP00000163717 antibody OTTHUMP00000163720 antibody OTTHUMP00000163724 antibody OTTHUMP00000163725 antibody OTTHUMP00000165062 antibody OTTHUMP00000165063 antibody OTTHUMP00000165064 antibody OTTHUMP00000206552 antibody OTTHUMP00000206582 antibody
数据库入口号Swiss-Prot#:Q14790
Uniprot
Q14790
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Caspase-8 on different lysates using anti-Caspase-8 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.
ICC staining Caspase-8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Caspase-8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Caspase-8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.
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et al,A study on mechanism of SIRT3 inducing endocrine drug resistance in breast cancer via deacetylating YME1L1
, (2024),
PMID:
Yes
