产品详情
产品名称Tubulin beta-III Rabbit mAb
克隆号SP06-00
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名beta 3 tubulin antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody
数据库入口号Swiss-Prot#:Q13509
Uniprot
Q13509
计算分子量50 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Tubulin beta-III on different lysates using anti-Tubulin beta-III antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: SH-SY-5Y
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.
Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
Yes
