产品详情
产品名称GFP Rabbit mAb
克隆号SP069-0
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC
免疫原描述recombinant protein
标记Unconjugated
别名GFP antibody
Green fluorescent protein antibody
yfp antibody
数据库入口号Swiss-Prot#:
计算分子量26 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:10,000
IHC: 1:500-1:1,000
ICC: 1:500-1:1,000
Western blot analysis of GFP on K562 cells transfected with GFP using anti-GFP antibody at 1/10,000 dilution.
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
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