产品详情
产品名称Heme Oxygenase 1(HO-1) Rabbit mAb
克隆号SP08-07
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB IHC ICC/IF IP FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名32 kD antibody
bK286B10 antibody
D8Wsu38e antibody
heat shock protein 32 kD antibody
heat shock protein 32kD antibody
Heat shock protein antibody
Heme oxygenase (decycling) 1 antibody
Heme oxygenase 1 antibody
Hemox antibody
HMOX 1 antibody
Hmox antibody
Hmox1 antibody
HMOX1_HUMAN antibody
HO 1 antibody
HO antibody
HO-1 antibody
HO1 antibody
Hsp32 antibody
数据库入口号Swiss-Prot#:P09601
Uniprot
P09601
计算分子量33 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB 1:1000~1:5000
IHC 1:1000~1:10000
ICC/IF 1:100~1:500
IP 1:50
FC 1:50
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HO-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat spleen Lane 2: Mouse spleen Lane 3: A549 Predicted band size: 33kDa Observed band size: 33kDa
Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
Yes
