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位置: 首页 > 重组兔单抗 > MMP14 Rabbit mAb

MMP14 Rabbit mAb #48761

是否有货: Yes
产品详情

产品名称MMP14 Rabbit mAb

克隆号3-F7

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

纯化Affinity-chromatography

应用WB, ICC/IF, IHC, IP, FC

种属反应性Human;Mouse;Rat

免疫原描述A synthesized peptide derived from human MMP14

标记Unconjugated

别名Matrix metallopeptidase 14 (membrane inserted) antibody
Matrix metalloproteinase 14 antibody
Matrix metalloproteinase-14 antibody
Membrane type 1 matrix metalloproteinase antibody
Membrane type 1 metalloprotease antibody
Membrane type matrix metalloproteinase 1 antibody
Membrane-type matrix metalloproteinase 1 antibody
Membrane-type-1 matrix metalloproteinase antibody
MMP 14 antibody
MMP X1 antibody
MMP-14 antibody
MMP-X1 antibody
Mmp14 antibody
MMP14_HUMAN antibody
MMPX1 antibody
MT MMP 1 antibody
MT-MMP 1 antibody
MT1 MMP antibody
MT1-MMP antibody
MT1MMP antibody
MTMMP 1 antibody
MTMMP1 antibody

数据库入口号Swiss-Prot#:P50281

Uniprot P50281

计算分子量66 kDa

实际分子量56 kDa

配方Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
WB 1:1000-1:2000; IHC 1:100-1:200; ICC/IF 1:50-1:200; IP 1:20-1:50; FC 1:20-1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
ICC staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: MMP14 Rabbit mAb at 1/1k dilution
Lane 1 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 56 kDa
Exposure time: 4 seconds
All lanes: MMP14 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse heart lysates
Lane 2 : Mouse skin lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat skin lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 56 kDa
Exposure time: 5 seconds
All lanes:MMP14 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : MMP14 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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