产品详情
产品名称MMP14 Rabbit mAb
克隆号3-F7
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化Affinity-chromatography
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述A synthesized peptide derived from human MMP14
标记Unconjugated
别名Matrix metallopeptidase 14 (membrane inserted) antibody Matrix metalloproteinase 14 antibody Matrix metalloproteinase-14 antibody Membrane type 1 matrix metalloproteinase antibody Membrane type 1 metalloprotease antibody Membrane type matrix metalloproteinase 1 antibody Membrane-type matrix metalloproteinase 1 antibody Membrane-type-1 matrix metalloproteinase antibody MMP 14 antibody MMP X1 antibody MMP-14 antibody MMP-X1 antibody Mmp14 antibody MMP14_HUMAN antibody MMPX1 antibody MT MMP 1 antibody MT-MMP 1 antibody MT1 MMP antibody MT1-MMP antibody MT1MMP antibody MTMMP 1 antibody MTMMP1 antibody
数据库入口号Swiss-Prot#:P50281
Uniprot
P50281
计算分子量66 kDa
实际分子量56 kDa
配方Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:1000-1:2000; IHC 1:100-1:200; ICC/IF 1:50-1:200; IP 1:20-1:50; FC 1:20-1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
ICC staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: MMP14 Rabbit mAb at 1/1k dilutionLane 1 : Human spleen lysate Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 66 kDa Observed band size: 56 kDaExposure time: 4 seconds
All lanes: MMP14 Rabbit mAb at 1/1k dilutionLane 1 : Mouse heart lysates Lane 2 : Mouse skin lysates Lane 3 : Rat heart lysates Lane 4 : Rat skin lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 66 kDa Observed band size: 56 kDaExposure time: 5 seconds
All lanes:MMP14 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : MMP14 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.
如果您使用该产品48761发表了文章,请通知我们,让我们可以引用您的文献。