产品详情
产品名称CD3 epsilon Rabbit mAb
克隆号SY0239
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, IHC, IP, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CD3 epsilon antibody CD3e antibody CD3e antigen epsilon polypeptide (TiT3 complex) antibody CD3E antigen epsilon polypeptide antibody CD3E antigen, epsilon subunit antibody CD3e molecule epsilon antibody CD3e molecule, epsilon (CD3 TCR complex) antibody CD3e molecule, epsilon (CD3-TCR complex) antibody CD3E_HUMAN antibody IMD18 antibody T cell antigen receptor complex epsilon subunit of T3 antibody T cell surface antigen T3/Leu 4 epsilon chain antibody T cell surface glycoprotein CD3 epsilon chain antibody T-cell surface antigen T3/Leu-4 epsilon chain antibody T-cell surface glycoprotein CD3 epsilon chain antibody T3E antibody TCRE antibody
数据库入口号Swiss-Prot#:P07766
Uniprot
P07766
计算分子量23 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC:1:50-1:200
FC: 1:50-1:100
Western blot analysis of CD3 epsilon on different lysates using anti-CD3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Human thymus
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3 epsilon antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with CD3 epsilon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
All lanes : CD3 epsilon Rabbit mAb at 1/2k dilution
Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : CD3 epsilon knockout Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck.
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