alpha smooth muscle Actin Rabbit mAb#48785
评价
Yes
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with alpha smooth muscle Actin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: alpha smooth muscle Actin Rabbit mAb at 1/1k dilutionLane 1 : 293 whole cell lysates Lane 2 : A549 whole cell lysates Lane 3 : C2C12 whole cell lysates Lane 4 : PC12 whole cell lysates Lane 5 : C6 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 42 kDa Observed band size: 42 kDaExposure time: 6 seconds
All lanes:alpha smooth muscle Actin Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : alpha smooth muscle Actin knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence alpha smooth muscle Actin antibody (48785) ICC/IF staining of alpha smooth muscle Actin in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48785 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
