产品详情
产品名称GSK3 beta Rabbit mAb
克隆号SY28-03
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human;Mouse
免疫原描述recombinant protein
标记Unconjugated
别名Glycogen Synthase Kinase 3 Beta antibody Glycogen synthase kinase-3 beta antibody GSK 3 beta antibody GSK-3 beta antibody GSK3B antibody GSK3B_HUMAN antibody GSK3beta isoform antibody Serine/threonine-protein kinase GSK3B antibody
数据库入口号Swiss-Prot#:P49841
Uniprot
P49841
计算分子量46 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of GSK3 beta on different lysates using anti-GSK3 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: NIH/3T3 Lane 4: PC12
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-GSK3 beta antibody. Counter stained with hematoxylin.
ICC staining GSK3 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GSK3 beta in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GSK3 beta in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with GSK3 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer��s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer��s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer��s disease.
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et al,Grass carp (Ctenopharyngodon idellus) SHP2 suppresses IFN I expression via decreasing the phosphorylation of GSK3 beta in a non-contact manner
, (2021),
PMID:
34265416