产品详情
产品名称Bim Rabbit mAb
克隆号SU0318
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC, IHC, IP
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名BCL2 like 11 antibody B2L11_HUMAN antibody BAM antibody Bcl 2 interacting protein Bim antibody Bcl 2 related ovarian death agonist antibody Bcl-2-like protein 11 antibody BCL2 interacting mediator of cell death antibody BCL2 like 11 (apoptosis facilitator) antibody BCL2 like protein 11 antibody Bcl2-interacting mediator of cell death antibody Bcl2-L-11 antibody Bcl2l11 antibody BIM alpha6 antibody BIM antibody BIM beta6 antibody BIM beta7 antibody BimEL antibody BimL antibody BOD antibody
数据库入口号Swiss-Prot#:O43521
Uniprot
O43521
计算分子量22/16/13 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Jurkat Lane 3: A431
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bim antibody. Counter stained with hematoxylin.
ICC staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Bim in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Bim in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.
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